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Carolus Vitalis
PhD Candidate · Biomedical Engineering · University of Colorado Boulder

Ph.D. candidate in Biomedical Engineering at the University of Colorado Boulder developing an AI-enabled platform for the computational design and experimental validation of novel transcriptional units in synthetic biology. Research spans biological design automation, SBOL-based data standards, machine learning for genetic part curation, and wet-lab characterization, with seven peer-reviewed publications and three manuscripts under review. NSF NRT Fellow in Integrated Data Science; experienced in interdisciplinary collaboration, curriculum development, and graduate mentoring.

Education

University of Colorado Boulder
PhD Candidate in Biomedical Engineering · Expected graduation: Summer 2026
  • Thesis: "AI-Enabled Platform for the Creation and Optimization of Novel DNA Transcriptional Units in Synthetic Biology"
  • Committee: Dr. Chris Myers, Dr. Thomas Cech, Dr. Jerome Fox, Dr. Ryan Layer, Dr. Aaron Adler
  • NSF NRT Graduate Training Program in Integrated Data Science
  • Interdisciplinary Quantitative Biology Certification
University of Colorado Boulder
M.Sc. in Biomedical Engineering
University of Chile
P.Eng. in Molecular Biotechnology Engineering · Graduated with Highest Distinction
  • Thesis: "DNA Assembly Automation to Speed Up the Design, Build, Test, Learn Cycle"
University of Chile
B.Sc. (Hons.)
University of Ottawa
Exchange Student
  • Techniques in Biomedical Engineering, Plant Developmental Biology, and Cellular Regulation and Control

Fellowships, Funding & Honors

Graduate Leadership Award. Biomedical Engineering Program, University of Colorado Boulder
  • Recognized for authoring a Biomedical Engineering teaching assistant mentoring handbook, strengthening TA training, and serving as President of the BME Graduate Student Organization, where I implemented a graduate student mentoring program for incoming students
Sigma Xi, The Scientific Research Honor Society. University of Colorado
Conference Travel Grant. University of Colorado Boulder
Supported travel to IWBDA 2025 and SEED 2025
Biomedical Engineering Teaching Fellowship. University of Colorado Boulder
NSF NRT Fellowship in Integrated Data Science. National Science Foundation
Dean's Future Leaders Fellowship. University of Colorado Boulder
Dean's Fellowship for Excellence. University of Colorado Boulder
Graduate School Dean's Fellowship. University of Colorado Boulder
Interdisciplinary Quantitative Biology Ph.D. Certificate. University of Colorado Boulder
SBOL Industrial Summer Internship. SBOL Industrial Consortium
Emerging Leaders in the Americas Program. Canadian Government
Academic Merit Fellowship. Municipality of Navidad
Atención Económica Scholarship. University of Chile
Gratuidad Scholarship. Ministry of Education of Chile
Eliodoro Cereceda Award. Internado Nacional Barros Arana
Best Biology Student of the Generation
Centenario Scholarship. Internado Nacional Barros Arana
Chile VA. Comisión Nacional de Investigación Científica y Tecnológica
One of 100 national top students of the generation

Publications

Journal Articles

Vitalis C, Vidal G, Samineni SP, Fontanarrosa P, Myers CJ. "A Framework for a Standard-Enabled FAIR Data Management Workflow for Synthetic Biology." ACS Synthetic Biology. 2026.

Buecherl L, Buson FX, Hau Sørensen G, Vitalis C, Kubaczka E, Vidal G, Bartley B, Ho Y, Misirli G, Gorochowski TE, Beal J, Myers CJ, Vaidyanathan P. "A Decade of SBOL Visual: Growing Adoption of a Diagram Standard for Engineering Biology." ACS Synthetic Biology. 2025. doi:10.1021/acssynbio.5c00417

Vitalis C, Wenzel T. "Leveraging Interactions in Microfluidic Droplets for Enhanced Biotechnology Screens." Current Opinion in Biotechnology. 2023. doi:10.1016/j.copbio.2023.102966

Samineni SP, Vidal G, Vitalis C, Yáñez Feliú G, Rudge TJ, Myers CJ, Mante J. "Experimental Data Connector (XDC): Integrating the Capture of Experimental Data and Metadata Using Standard Formats and Digital Repositories." ACS Synthetic Biology. 2023;12(4):1364–1370. doi:10.1021/acssynbio.2c00669

Vidal G, Vitalis C, Muñoz-Silva M, Castillo-Passi C, Yáñez Feliú G, Federici F, Rudge TJ. "Accurate Characterization of Dynamic Microbial Gene Expression and Growth Rate Profiles." Synthetic Biology. 2022;7(1). doi:10.1093/SYNBIO/YSAC020

Vidal G, Vitalis C, Rudge TJ. "LOICA: Integrating Models with Data for Genetic Network Design Automation." ACS Synthetic Biology. 2022;11(5):1984–1990. doi:10.1021/acssynbio.1c00603

Yáñez Feliú G, Earle Gómez B, Codoceo Berrocal V, Muñoz Silva M, Nuñez IN, Matute TF, Arce Medina A, Vidal G, Vitalis C, Dahlin J, Federici F, Rudge TJ. "Flapjack: Data Management and Analysis for Genetic Circuit Characterization." ACS Synthetic Biology. 2021;10(1):183–191. doi:10.1021/acssynbio.0c00554

Book Chapters

Vidal G, Vitalis C, Guillén J, Beal J. "Standardized Golden Gate Assembly Metadata Representation Using SBOL." In: Golden Gate Cloning, Ch. 6. Springer Nature; 2024. doi:10.1007/978-1-0716-4220-7_6

Vitalis C, Yáñez Feliú G, Vidal G, Muñoz Silva M, Matúte T, Núñez I, Federici F, Rudge TJ. "Flapjack: Data Management and Analysis for Genetic Circuit Characterization." In: Synthetic Biology, 2nd Ed., Ch. 23. Springer Nature; 2024. doi:10.1007/978-1-0716-3658-9_23

Vidal G, Vitalis C, Matúte T, Núñez I, Federici F, Rudge TJ. "Genetic Network Design Automation with LOICA." In: Synthetic Biology, 2nd Ed., Ch. 22. Springer Nature; 2024. doi:10.1007/978-1-0716-3658-9_22

Manuscripts Under Review and in Preparation

Vitalis C, Sherstiukova T, Kretzschmar I, Vidal G, Martinez M, Seaman L, Vaiana CA, Myers C. "Excel-to-SBOL Converter 2: An Enhanced Standardized Data Capture Tool for Synthetic Biologists." Manuscript in preparation. 2026.

Vitalis C, Myers C. "BioPartCurator: Genetic Part Unraveling Through Machine-learning Algorithms." Manuscript in preparation. 2026.

Vitalis C, Myers C. "ExpressionCassetteBuilder: An AI-Enabled Platform for the Creation and Optimization of Novel DNA Transcriptional Units in Synthetic Biology." Manuscript in preparation. 2026.

Research Experience

Genetic Logic Lab. University of Colorado Boulder
Research Assistant · Dr. Chris Myers
  • Built ExpressionCassetteBuilder to automate the design of assembly-ready transcriptional units from natural language inputs, closing the gap between design intent and bench-compatible SBOL genetic constructs
  • Built BioPartCurator to address annotation inconsistency in genetic part databases, applying machine-learning-based extraction and verification to produce curated, reusable part libraries for downstream genetic design
  • Improved Excel-to-SBOL Converter v2 to enable FAIR data standards in laboratory practice, converting researcher-maintained spreadsheets into SBOL-compliant records for registry upload and cross-lab reuse
  • Contributed to BuildPlanner to automate the translation of SBOL genetic designs into bench-ready assembly protocols, streamlining the design-to-build transition in DBTL workflows
  • Developing wet lab validation experiments using dual reporter plasmids and plate reader assays to close the loop between design prediction and experimental characterization
  • Supported the Army Center for Synthetic Biology through data infrastructure and synthetic biology workflow development
Mars Desert Research Station. Hanksville, Utah
Analog Astronaut · Mission Leader · ASEN 5226 Medicine in Space and Surface Environments
  • Led a multidisciplinary crew during simulated extravehicular activities, rover operations, emergency triage, and mission planning in an analog Mars habitat
  • Coordinated crew schedules, safety protocols, and operational objectives during a field deployment focused on space medicine and surface operations
Reimagining Biocatalytic Systems Lab. University of Colorado Boulder
Research Rotation · Dr. Jerome Fox
  • Worked with cyanobacterial chassis, including transformation of Synechococcus sp. PCC 7002
  • Designed plasmids to adapt T7-based expression around toxicity constraints in cyanobacterial genetic engineering
Living Matter Lab. University of Colorado Boulder
Research Rotation · Dr. Mirela Alistar
Institute for Biological and Medical Engineering. Pontifical Catholic University of Chile
Research Assistant · Dr. Tobias Wenzel
  • Researched microfluidics and developed the curriculum for the Synthetic Biology and Prototyping course
Twist Bioscience
Research Intern · SBOL Industrial Consortium
  • Extended Flapjack and LOICA to full SBOL3 compliance
Interdisciplinary Computing and Complex BioSystems. Newcastle University, UK
Research Collaboration · Dr. Timothy J. Rudge
  • Contributed to front-end development of Flapjack and to data visualization and simulation pipelines for LOICA
Institute for Biological and Medical Engineering. Pontifical Catholic University of Chile
Research Project · Dr. Timothy J. Rudge
  • Delivered a DNA assembly automated protocol using a liquid-handling robot (Opentrons OT-2)
  • Python programming, thermocycler handling for PCR execution, bacterial transformation, and Molecular Biology protocols
Centre of Molecular Biology in Plants. University of Chile
Traineeship · Dr. Claudia A. Stange
  • Molecular Biology laboratory techniques: vector and primer generation, culture media preparation, and PCR

Teaching Experience

Co-Instructor, Engineering Genetic Circuits
University of Colorado Boulder · Upper-division undergraduate and graduate course in synthetic biology and genetic circuit design
  • Delivered lectures and hands-on labs on genetic circuit design, DNA assembly techniques, and synthetic biology principles for mixed undergraduate/graduate enrollment
  • Authored complete lab guides, problem sets, and assignments; graded exams with calibrated rubrics and returned timely feedback
  • Integrated widely used synthetic biology tools, including SBOL, SynBioHub, and iBioSim, into design, build, and test workflows
  • Coached project teams through full design cycles from genetic part characterization to circuit-level validation
  • Collaborated with course instructor on curriculum planning, learning objectives, and assessment design
Lab Demo and Open Lecture
Synthetic Biology Workshop · University of Colorado Boulder
  • Conducted practical demonstrations on laboratory automation and synthetic biology workflows using the Opentrons OT-2 liquid-handling robot
Course Developer, Engineering Genetic Circuits
Coursera · Online adaptation of the University of Colorado Boulder course
  • Helped develop the online version of the course, integrating advanced computational tools for genetic design into a self-paced format
  • Designed and quality-assured quizzes and programming exercises to enhance student understanding of circuit engineering concepts
Co-Instructor, Synthetic Biology and Prototyping
Pontifical Catholic University of Chile · Undergraduate engineering course
  • Delivered lectures covering synthetic biology design principles, molecular biology techniques, and engineering approaches to biological systems
  • Designed comprehensive teaching materials: lectures, quizzes, lab guides, projects, and computational exercises for interdisciplinary learning
  • Led hands-on lab sessions on DNA assembly and genetic circuit design
  • Mentored students on research and project development, guiding application of synthetic biology methodologies to real-world problems
Open Lecture
Tan Tári Open Academy · University of Chile
  • Introduced synthetic biology concepts like DNA assembly and circuit optimization to interdisciplinary audiences of students and professionals
Teaching Assistant, Instrumentation
University of Chile · Undergraduate engineering course
  • Led hands-on workshops on designing and building hardware with Arduino microcontrollers for medical and scientific applications
  • Taught programming fundamentals, sensor integration, and real-time data acquisition and analysis
Teaching Assistant, Plant Physiology
University of Chile · Undergraduate biology course
  • Guided students through molecular and cellular processes underlying plant growth and development
  • Clarified complex topics on plant hormone regulation and stress responses during office hours
Teaching Assistant, Optimization
University of Chile · Undergraduate mathematics and engineering course
  • Supported students in mathematical optimization principles applied to biological and engineering systems
  • Developed supplementary Python materials for linear and non-linear optimization, including ready-to-run scripts

Mentoring

Indiana Kretzschmar. Undergraduate Research · University of Colorado Boulder
Taya Sherstiukova. Undergraduate Research · University of Colorado Boulder
Duncan Brit. Undergraduate Research · University of Colorado Boulder
William Dravenstott. Undergraduate Research · University of Colorado Boulder
Dylan Smith. Undergraduate Research · University of Colorado Boulder
Francisca Becker. Undergraduate Research · Pontifical Catholic University of Chile
Pablo Caballero. Undergraduate Research · Pontifical Catholic University of Chile
Liliana Peña. Undergraduate Research · Pontifical Catholic University of Chile

Academic Service

CU Boulder Biomedical Engineering Graduate Student Organization. Boulder, USA
President (2025) · Vice President (2024)
  • Implemented a peer mentoring program connecting incoming Biomedical Engineering graduate students with current students to support transition, community building, and long-term student relationships
Biomedical Engineering Program. University of Colorado Boulder
Teaching Assistant Lead
  • Led training and mentoring for Biomedical Engineering teaching assistants across the program
  • Authored a BME teaching assistant handbook to standardize expectations, clarify teaching practices, and support new teaching assistants
  • Served as a program-level resource for teaching assistants, supporting grading practices, office hours, lab support, student communication, and course logistics
International Workshop for BioDesign Automation (IWBDA). Boulder, USA
Program Chair
NASA Colorado Space Grant Consortium (COSGC). Boulder, USA
Judge · Undergraduate Space Research Symposium
  • Evaluated undergraduate space research presentations for the 2026 COSGC Undergraduate Space Research Symposium, a NASA-funded statewide space education and research program
International Workshop for BioDesign Automation (IWBDA). Worcester, USA
Publication Chair
ACS Synthetic Biology
Guest Editor · "Bio Innovation via Synthetic Biology" special issue
CU Boulder International Student Services. Boulder, USA
Graduate Student Mentor · 4 mentees
International Workshop for BioDesign Automation (IWBDA). Boston, USA
Publication Chair · Session Chair: Automating and Connecting the DBTL Cycle
International Workshop for BioDesign Automation (IWBDA). Boston, USA
Workshop Chair · Session Chair: Sequence Manipulation and Gene Expression
Synthetic Biology Open Language (SBOL)
Editor (2023–Present) · Member (2021–Present)
International Genetically Engineered Machine Competition (iGEM)
Member, Engineering Committee
  • Contributed to committee-level work on synthetic biology engineering practice, measurement and calibration standards, biological device predictability, and design-build-test-learn guidance for the global iGEM competition
Asociación Nacional de Estudiantes de Ingeniería en Biotecnología (ANEIB). Chile
President, Chilean National Association of Biotechnology Engineering Students (2021–2023) · Ambassador (2018–2020)
Congreso Nacional de Estudiantes de Ingeniería en Biotecnología (CONEIB). Virtual
Chairman, Organizing Committee
  • Contacted speakers worldwide; organized a conference attended by 300 participants

Conferences and Invited Presentations

IQ Biology QED Talk. Boulder, USA
  • Speaker: "The Art of the Scientific Poster: Typography, Color, Layout, and Visual Storytelling"
International Workshop for BioDesign Automation (IWBDA). Boston, USA
  • (Talk) BuildPlanner
  • (Talk) Excel-SBOL Converter Version 2
Synthetic Biology: Engineering, Evolution & Design (SEED). Texas, USA
  • (Poster) Synthetic Biology Open Language: Standardizing Design Representation and Enabling Interoperability
TEDxCU 2025. Boulder, USA
  • Invited Speaker: "One Nucleotide at a Time: AI and the Future of Synthetic Biology"
International Workshop for BioDesign Automation (IWBDA). Boston, USA
  • (Talk) PUMA: Promoter Unraveling through Machine-learning Algorithms
  • (Workshop) SBOL Notebooks
IQ Biology Symposium. Boulder, USA
  • (Poster) PUMA: Promoter Unraveling through Machine-learning Algorithms
IQ Biology Donors Presentation. Boulder, USA
  • (Poster) Designing, Characterizing, and Optimizing Synthetic Genetic Circuits
Synthetic Biology: Engineering, Evolution & Design (SEED). Georgia, USA
  • Representing the Synthetic Biology Open Language
Hackathons on Resources for Modeling in Biology (HARMONY). London, UK
  • (Talk) PUMA: Promoter Unraveling through Machine-learning Algorithms
  • (Workshop) SBOL Visual Hackathon
  • (Workshop) SBOL Visual Font
Army Center for Synthetic Biology: Annual Review Meeting. Austin, USA
  • A Data Management Plan for the Army Center for Synthetic Biology
Computational Modelling in Biology Network (COMBINE). Connecticut, USA
  • Representing the Synthetic Biology Open Language
International Workshop for BioDesign Automation (IWBDA). Boston, USA
  • (Workshop) Programmatic genetic design automation using LOICA
  • (Talk) A Report on SynBio Data Management Practices
  • (Talk) Software for Synthetic Biology Workflows: How to Improve Your Productivity
  • (Talk) PUDU: Simple Liquid Handling Robot Control for Synthetic Biology Workflows
Synthetic Biology: Engineering, Evolution & Design (SEED). California, USA
  • (Workshop) Software for Synthetic Biology Workflows: How to Improve Your Productivity and Impact
Hackathons on Resources for Modeling in Biology (HARMONY). Washington DC, USA
  • SBOL Hackathon · SEED Tutorial Preparation
Hackathons on Resources for Modeling in Biology (HARMONY). Washington DC, USA
  • (Talk) Connecting Up the DBTL Cycle Using SBOL3
Synthetic Biology: Engineering, Evolution & Design (SEED). Washington DC, USA
  • (Workshop) Software Tools Workshop: Describing a complete workflow of the DBTL cycle
  • (Poster) LOICA 1.2: Genetic Network Design Automation for Spatio-Temporal Patterns
Computational Modelling in Biology Network (COMBINE). Virtual
  • (Talk) LOICA: Logical Operators for Integrated Cell Algorithms
International Workshop for BioDesign Automation (IWBDA). Virtual
  • (Workshop) Flapjack Workshop
  • (Talk) LOICA: Logical Operators for Integrated Cell Algorithms
Synthetic Biology: Engineering, Evolution & Design (SEED). Virtual
  • (Talk) Flapjack: A Data Management and Analysis Tool for Genetic Circuit Characterization
1st Instituto de Ingeniería Biológica y Médica Networking Session. Santiago, Chile
  • (Talk) Flapjack: A Data Management and Analysis Tool for Genetic Circuit Characterization
Computational Modelling in Biology Network (COMBINE). Virtual
  • (Talk) Flapjack: A Data Management and Analysis Tool for Genetic Circuit Characterization

Skills

Wet Lab Skills
Microbiology, bacterial and mammalian cell culture, bacterial transformation, PCR, primer and vector design, DNA assembly, plasmid design, culture media preparation, thermocycler handling, microplate reader assays, microscopy
Synthetic Biology
Genetic circuit design, transcriptional unit design, biological part characterization, design-build-test-learn workflows, Golden Gate assembly metadata, SBOL-based design representation, synthetic biology data standards
Synthetic Biology Software and Platforms
BioPartCurator, ExpressionCassetteBuilder, Excel-to-SBOL Converter v2, BuildPlanner, Flapjack, LOICA, SynBioHub, iBioSim
Laboratory Automation
Opentrons OT-2, automated liquid handling, protocol implementation, experimental data capture
Computational Skills
Python, Bash, LaTeX, data analysis, workflow automation, machine learning for genetic part discovery and annotation verification
Space and Field Operations
Analog mission leadership, EVA planning, rover operations, emergency triage, field safety protocols
Languages
English and Spanish (Bilingual), Italian (Basic), Latin (Basic)

Certifications and Field Training

Basic Life Support: CPR & AED
American Heart Association
Utah Off-road Vehicle
Utah OHV Education
Wilderness First Aid
University of Colorado Boulder
Alberta RNA Research and Training Institute
University of Lethbridge · Dual Use Research of Concern
Python Basics
University of Michigan
Machine Learning Feature Selection in Python
Coursera Project Network
Class Creation in Object-Oriented Python
Coursera Project Network
Processing Data with Python
Coursera Project Network
Bank Factoring
Banco Consorcio

Technology Transfer

Smartwatering
Faculty of Economy and Business · University of Chile
  • First place in the Sustainable Entrepreneurship course before the Chilean Government Jury
Iodine Supplementation in Plants through Bioaccumulation
OpenLab · University of Chile · Project Director
  • Delivered a solution to address iodine deficiency in the food supply

Software Engineering Experience

ExpressionCassetteBuilder. Genetic Logic Lab, University of Colorado Boulder
Lead Developer
  • Python backend with a cross-platform GUI; core design engine uses a fine-tuned large language model to generate assembly-ready SBOL transcriptional units from natural language design requests
  • Accepts chassis selection, assembly standard (MoClo, BioBrick, or Free Assembly), and optional SynBioHub authentication; outputs SBOL files with human- and robot-readable lab assembly protocols
BioPartCurator. Genetic Logic Lab, University of Colorado Boulder
Lead Developer
  • Python backend with a cross-platform GUI; applies classification and named entity recognition to annotate and verify genetic parts extracted from GenBank records
  • Outputs curated part data as CSV and Excel files directly compatible with Excel-to-SBOL for downstream SBOL library construction
Excel-to-SBOL Converter v2. Genetic Logic Lab, University of Colorado Boulder
Lead Developer
  • Python backend with a cross-platform GUI; outputs SBOL2 and SBOL3; BSD-3 Clause license; deployed on GitHub
  • Four-template architecture (Resources, Strains, Sample Design, Study) with VBA macro validation and a layered error-reporting framework distinguishing critical errors from non-fatal warnings
  • SynBioHub REST API integration for authentication and duplicate prevention; validated at DARPA TELLUS scale with 1,246+ parts
BuildPlanner. Genetic Logic Lab, University of Colorado Boulder
Contributor · Validation
  • Tool takes SBOL assembly design specifications as input and outputs human- and robot-readable lab assembly protocols; contributed validation logic for SBOL structure and assembly metadata
Flapjack. Interdisciplinary Computing and Complex BioSystems, Newcastle University, UK
Contributor · Front-End Development
  • Contributed UI components to this web-based platform for linking plate reader measurements to SBOL-described designs and experimental metadata across DBTL cycles
LOICA. Interdisciplinary Computing and Complex BioSystems, Newcastle University, UK
Contributor · Visualization and Simulation
  • Built genetic network graph visualizations using NetworkX and Matplotlib; ran continuous and stochastic ODE simulations for experimental validation of genetic circuit models
Banco Consorcio
Project Manager · Virtual Bank Deployment
  • Delivered a brand-new nationwide web-based customer attention system

Volunteering

PreuPortaleanos
IT Manager
Microsoft Answers
IT Support